Biological activities of dithiocarbazate schiff base derivatives on normal and diabetic rats / Fathi Farag Mughrabi

The present study was performed to evaluate the acute
toxicity, anti-ulcer, wound healing, antioxidant and
antibacterial activities of a Schiff base namely benzyl
N’-(indol-3-ylmethylidene)-hydrazinecarbodithioate
(BIHC) and its metal complexes, BIHCZn and BIHCNi. The
three compounds namely BIHC, BIHCZn and BIHCNi are
subsequently collectively referred to as BIHCD. Acute and
subchronic toxicity investigation of BIHCD did not show any
signs or symptoms of toxicity and all animals remained alive.
Blood biochemistry, hematology, and histopathology analysis did not show any significant differences between control
and treated groups. BIHCD appears to be safe and no drug-related toxicity was detected even at the highest dose
investigated (400mg/kg). Antiulcer activity of BIHCD against ethanol-induced gastric ulcer was studied. Gastric juice
was collected for the determination of pH and mucous weight. Grossly, the ulcer control group exhibited severe mucosal
injury, whereas pre-treatment with either derivative compounds or Omeprazole resulted in significant protection of
gastric mucosal injury. Flattening of gastric mucosal folds was also observed in rats pretreated with BIHCD. Histological
studies of the gastric wall of ulcer control group revealed severe damage of gastric mucosa, along with oedema and
leucocytes infiltration of the submucosal layer compared to rats pre-treated with either BIHCD or Omeprazole where
there were marked gastric protection along with the reduction or absence of oedema and leucocytes infiltration of the
submucosal layer. BIHCD and its derivatives promote ulcer protection as ascertained by the comparative decreases in
ulcer areas, reduction of oedema and leucocytes infiltration of the submucosal layer. Topical application of BIHC and its
metal complexes on the wounds in normal and diabetic rats showed that wound dressed with BIHCD healed significantly
earlier than those treated with 10% Tween 20.