Lansium domesticum components profiling by using liquid chromatography - mass spectrometry

Lansium domesticum, locally known as "Dokong", found in the tropics regions of the world, predominantly in Thailand, Malaysia, Indonesia, Philippines and Vietnam. It is also used to alleviate some medial conditions as it possess antidiarrheal, antispasmodic, antipyretic, antimalarial and anti-inflammatory properties. In 2015, Agensi Antidadah Kebangsaan (AADK) Perlis discovered that the "Ketum" addicts are taking Lansium domesticum leaves extract as an alternative to "Ketum" extract due to "Ketum" scarcity after it is banned because of misuse and abuse. "Ketum" addicts claimed that both of the extracts have similar euphoric effect. However, there is insufficient information regarding compounds that may contribute to the euphoric effect in Lansium domesticum leaves extract. The objective of this study is to detect presence of mitragynine in Lansium domesticum leaves extract. Lansium domesticum leaves was extracted by using ethanol solvent (95% ethanol) and aqueous solvent (distilled water). Extracts were prepared using condensation method by rotary evaporator for ethanol extraction and lyophilisation method for aqueous extraction. Both extracts undergone chromatographic analysis by Liquid Chromatography- Mass Spectrometry Quadrupole - Time of Flight (LC-MS QTOF) system. The parameters tested were retention time, m/z value (mass-to­ charge ratio) and predicted structure. The results were compared with Mitragyna speciosa chromatographic analysis result. The results showed that mitragynine was absent in Lansium domesticum leaves extracts. The euphoric effect claimed may be due to other compounds present in the leaves extract.