Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak

Ismail, Normah and Abd Razak, Nurulain (2014) Characterization and purification of protease extracted from two maturity stages of ‘Noni’ (Morinda citrifolia L.) fruit / Normah Ismail and Nurulain Abd Razak. Scientific Research Journal, 11 (2). pp. 1-16. ISSN 1675-7009

Official URL: https://srj.uitm.edu.my/

Abstract

Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5). The crude extract was partially purified by acetone precipitation method followed by dialysis, gel filtration chromatography and freeze drying. Protein
concentrations, proteolytic activity, molecular weight distribution, pH stability, temperature stability and storage efficiency of the resulting protease were evaluated. The unripe and ripe noni fruit contains 0.65 and 0.35% protein, respectively. Molecular weight of the proteases from both stages ranged approximately between 3 to 28 kDa based on the SDS-PAGE results. The optimum
activity were at pH 7s and 6, temperatures of 40 and 50°C, respectively for proteases obtained from the unripe and ripe fruit. Analysis from the freeze dried protease indicated that protease from ripe noni fruits had higher protein concentration and specific activity compared to those from unripe fruit. However, it is more sensitive to pH and temperature and less stable during storage as it shows lower proteolytic activity compared to protease from unripe fruit. Based on its high proteolytic activity reaching up to 70.31 U/mg and storage stability (30% lost of activity), noni fruit could be an alternative source of plant protease.

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Ismail, Normah
norismel@salam.uitm.edu.my
Abd Razak, Nurulain
UNSPECIFIED
Subjects: Q Science > QK Botany > Rubiaceae (Madder). Morinda citrifolia
Q Science > QP Physiology > Animal biochemistry > Proteolytic enzymes. Proteases
Divisions: Universiti Teknologi MARA, Shah Alam > Research Management Centre (RMC)
Journal or Publication Title: Scientific Research Journal
UiTM Journal Collections: UiTM Journal > Scientific Research Journal (SRJ)
ISSN: 1675-7009
Volume: 11
Number: 2
Page Range: pp. 1-16
Keywords: protease, noni, Morinda citrifolia L., proteolytic activity, acetone precipitation
Date: 2014
URI: https://ir.uitm.edu.my/id/eprint/12429
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