Pollen analysis of heterotrigona itama honey

This study focused on pollen analysis in Heterotrigona itama (H. itama) honey harvested from Lenggong, Perak (Malaysia). Pollen analysis is frequently used to determine the environmental context of archaeological artefacts (Twiddle, 2012). The study of pollen analysis in honey calculated the abundance of pollen grains in H. itama honey and determine the preferred flowers chose by H. itama towards choosing flower for its food. Previously, pollen analysis was used to solve the problem of mad-honey intoxification (Cagli et al., 2009), analyse the presence of chemical and pollen in Silver Lake (Gordon, 1966) and related the theoretical relationship between vegetational percentages and percentage of pollen in sediment (Crowell, 1978). The principal methods involved in pollen analysis are qualitative and quantitative methods (Moar, 1985). The quantitative method involves calculation of relative pollen frequencies from total count of pollen (Moar, 1985). In contrast, the qualitative method consists of morphological observation of pollen presence honey (Hamid et al., 2015)