The potential of marine endophyte fungi extracts in prevention of UVB irradiation against fibroblast cell lines

Ultraviolet radiation, particularly UVB (290-320 run) is the major causes of many harmful effects, including photoaging, cutaneous inflammation, sunburn, skin cancer as well as able to cause protein oxidation, cellular and DNA damages. Photochemoprevention by using natural antioxidant in protecting human skin against UVB damage is receiving increased attention. Therefore, in this study, the effect of marine endophytic fungi (MV, CN and ED) that colonise in local seaweeds ( Graci/aria coronopifolia J. Agard, Graci/aria arcuata Zanardini, and Acanthophora spicifera) respectively on UVB induce damage of human fibroblast BJ cell lines were investigated. At density of 2x 104 of fibroblast BJ cell lines were seeded on 96 well plate in triplicate manner. The cell lines were exposed to the UVB radiation for 60 seconds in order to induce DNA damage and were tested using different concentrations of marine endophytic fungi extracts were added in each wells for 24 hours. For photoprotective activity, the cell lines were treated with different concentration of extracts and then exposed to UVB radiation. From this study, the result exhibited no photoprotective and reduce DNA damage effects against fibroblast BJ cell lines as 60 seconds of UVB irradiation time exposure were inadequate to damage fibroblast BJ cell lines. Therefore, the ability of these three marine endophytic fungi extracts which were CN, MV and ED in reducing and exhibited were unable to be proven in this study.