Attachment of Pseudomonas aeruginosa NR.22 (Ps.NR.22) on ceramic for filtration of crude oil in water mixture: article

Pseudomonas aeruginosa NR.22 (Ps.NR.22) was subcultured from pure strain isolated by Nik Raikhan and Khairul Izwan has a Gram negative rod shaped bacteria that has potential to degrade the crude oil which consisted of SARA component. Crude oil from Dulang Field was homogenized by using Ultrasonic homogenizer. As to meet the first objective, Ps.NR.22 was attached to a ceramic membrane by using deadend method. The second objective was observed and analyzed by using comparison method between oil-water mixture that was filtered with no bacteria and with the presence of bacteria. The result was further examined by using ultraviolet-visible (UV-VIS) spectroscopy which resulted in a constant concentration which only differed by 0.86 % for the filtration without attachment of bacteria. The concentration has decreased by 22.43 % with the presence of bacteria. Another result was analyzed by Fourier Transform Infrared Spectroscopy (FTIR) which resulting in increment of degradation of crude oil from three bond to six bond for the filtration with the presence of bacteria. On the other hand, the morphological membrane characterization was done by digital microscope as it is revealed that the bacteria were actually degraded the crude oil and thus, the surface of membrane was clearer than the surface without bacteria. In conclusion, the oil-water mixture filtered with presence of bacteria was clearer compared to the product of ceramic membrane without bacteria. To achieve precise result, various species of bacteria need to be attached on the ceramic membrane in order to filter the crude oil to get the clearer water.