Determination of half maximal inhibitory concentration IC50 of hydrogen peroxide on fibroblast skin cell

Hydrogen peroxide can be used to promote the cell growth in the wound healing process if the concentration is ideal for the cell. In this research, the determination of half maximal inhibitory concentration of hydrogen peroxide in CRL-2522 fibroblast skin is conducted through MTT assay method. The CRL-2522 was cultured in a favourable media before proceed with the MTT assay. The positive control was treated with media while negative control was treated with 5% concentration of hydrogen peroxide. The hydrogen peroxide was diluted into a various concentration, such as 0.3%, 1 %, 1.25%, 2.3% and 3%. After the cell confluent in the 96-well plate, the different concentration of hydrogen peroxide was used as a treatment in each wells. In conclusion, hydrogen peroxide had a value of IC50 which is above 3% of the concentration.