Amplification of exon 3 of human patatin-like phospholipase domain containing 3 gene (PNPLA3) using polymerase chain reaction

Liver fibrosis is the accumulation of scar tissue in the liver. It takes place due to prolonged inflammation and in response of the liver towards injuries or diseases. The mutation of PNPLA3 gene from 10109 C>G in exon 3 may lead to liver fibrosis. This is associated with risk factors that are common in Malaysia such as CLD, NAFLD and excessive alcohol consumption. A set of primer with size of 355 bp that will amplify exon 3 of PNPLA3 gene has been successfully designed in the detection of the mutation of PNPLA3 gene even though the result shows absence of mutation. Future studies using the successfully designed primer can be conducted with people of high risk to develop liver fibrosis among Malaysian. By doing so, public may have better awareness on the genetic factors that may contribute to this disease among our population.