Inhibitory of Lawsonia inermis leaves extract on pancreatic enzymes: effect of drying temperature: article

Obesity is a serious and health risk as it is a major contributor towards chronic diseases and disability including hypertension, stroke, diabetes, cardiovascular and others. Therefore, this research aimed to discover the potential of Lawsonia inermis crude extract as a new and safe alternative lipase inhibitor to treat obesity by utilizes the natural sources, instead of using chemical drugs. The phenolic (gallic acid) and flavonoid (quecertin) compound in this plant are expected to promote inhibitory activity towards pancreatic lipase enzyme. The part of this plant selected was leaves. The extraction of leaves with distilled water were analyzed for phytochemical compounds through Fourier Transform Infrared Spectroscopy (FTIR), Total Flavonoid Content (TPC), Total Phenolic Content (TPC) and spectrophotometer. FTIR analysis of the extracts of L. inermis revealed the presence of hydroxyl functional group in leaves extracts. This validate the presence of phenolic compounds in the leaves extract for pancreatic lipase inhibition. The highest quantity TPC and TFC value were 93.24 mg of GAE/g of extract and 301.85 mg of QE/g of extract respectively at 60°C of drying temperature. The experimental maximum yield of TPC was 73.27 ±14 mg of GAE/g of extract for 60°C, 70°C and 80°C extracts of dried grinded leaves. Meanwhile, the experimental maximum yield of TFC was 275.70±47 mg of QE/g of extract for 60°C, 70°C and 80°C extracts of dried grinded leaves respectively Therefore, the leaves extracts have potential as an anti-obesity agent by inhibiting pancreatic lipase. Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. Pancreatic lipase inhibitory activity has been extensively used for the determination of potential efficacy of natural products as antiobesity agents. In this research, the method used to study the inhibition of gallic acid and quercetin in the sample L. inermis after drying at three differences temperature (60°C, 70°C and 80°C) in order to find out which is the optimal drying temperature. The potential role of plants which is L. inermis that have been investigated for their pancreatic lipase inhibitory activity. It was found that the highest percentage inhibition at 60°C of drying temperature in the 10 mg/mL of the extract.