Isolation and characterization of antimicrobial peptides from okara waste

Higher amount of okara waste produced from soy bean curd manufacturing is becoming global concern due to big disposal and environment problem. Utilization of okara can be very beneficial due to their high nutritive value as study stated that okara have potential to exert biological activities such as antimicrobial and antioxidant. This approach are milestone steps in reducing okara waste in directly become a big potential to use as source of natural antibiotic. The increasing dependency on antibiotic resulted in emerging of bacteria resistant is becoming a global concern. There is urgent need for innovation, for producing new natural resource antibiotic to prevent or lessen the antibiotic dependency. From previous studies, okara contains mostly crude fiber composed of cellulose, hemi cellulose, and lignin, about 25% protein, 10& lipd and other nutrients. Due to their its functional ingredient composition and health-promoting attributes such as therapeutic activity proved by previous researchers, this research aim is to isolate purify peptides from chosen byproduct waste which is okara; a soybean residue that contain its own nutritive value using enzymatic hydrolysis. The research also includes to determine antimicrobial activity and antioxidant activity of the isolated peptide bacteria using reverse-phase high performance liquid chromatography (HPLC) and Fourier-transform infrared (FTIR) were crucial strategic approaches. Based on the results, it was found that okara contain nitrogen represent about 69.43 % of protein. While HPLC found four unknown compounds in each trial that have same functional group tested by FTIR. Functional group of alkene, alkyne, alkane, alcohol was found in all four of the compounds (e.g. aspartame, glutamate, asparagine, serine) that have similar bands based on standard group band for after HPLC analysis.