Fouling-induced enzyme immobilization of alcohol dehydrogenase (ADH) using graphene oxide (GO) based polymer membrane

The use of Carbon dioxide (CO₂) to produce another beneficial product is a great advantage to humankind because CO₂ is harmful if the amount in the environment is not balance since it can cause greenhouse effect. Enzyme immobilization is use to produce methanol with alcohol dehydrogenase (ADH) as model enzyme in addition of nicotinamide adenine dinucleotide (NADH) as cofactor. The objectives of this research are to immobilize ADH in graphene oxide based polymer membrane by using fouling induced enzyme immobilization technique and to examine biocatalytic productivity of the enzymatic membrane reactor system. The conversion will be achieved using the optimum value of pressure (2bar), pH (7) and enzyme concentration (0.1 gL¯¹) to see how many cycle that the membrane can do to achieve the high yield of methanol. Based on the results, the amount of enzyme immobilize, permeate, retentate and washing are 1.36 mg, 0.7126 mg, 0.3114 mg, and 0.6171 mg respectively. For enzymatic reaction, conversion rate are 60% and 14% for reaction one and two respectively.