Cytotoxicity investigation of free schiff base ligands, PII and PDI against human hepatic cells (WRL-68)

This project involves in discovering the cytotoxic effect of free Schiff base ligands, PII and PDI on human hepatic cell lines. The goal was to prove that there is no cytotoxic effect of the compounds when tested on human hepatic cell lines (WRL-68), by using MTT assay. Five concentrations of each sample were tested against the hepatic cells (WRL-68) include 10 µg/ml, 50 µg/ml, 100 µg/ml, 500 µg/ml and 1000 µg/ml. The result revealed high cell viability at lowest concentration, 10 µg/ml that are 98.60% for PII and 80.17% for PDI before the percentage viability reduce as the concentration increases. At highest concentration, 1000 µg/ml, the cell viability are 30.42% for PII and 45.11 % for PDI. The IC50 for PII and POI are 1.25 mg/ml and 5.74 mg/ml respectively, indicated that higher concentration needed to inhibit cell activity of half population. As a conclusion, PII and POI has shown no cytotoxicity properties against human hepatic cells (WRL-68).