Development of hand sanitizer enhanced with green coffee beans extract

This research focused on Chlorogenic acids from green Arabica coffee beans extract Green coffee beans extract application is still low due to lack of research on its potential and benefits. Since green coffee beans claim to be rich in Chlorogenic acids, it will influence the potential benefits of the extract. Chlorogenic acids also has been proven to exhibit antibacterial activity on pathogenic bacteria. In response to utilize the ability of Chlorogenic acids from green coffee beans, this study conducted to develop sanitizer enhanced with green coffee beans extract which can be an alternative formulation compared to alcohol based. Green coffee beans extract was extracted by using Soxhlet extraction method and Chlorogenic acids was analyses by using UV-Vis spectroscopy. Central Composite design of response surface methodology (RSM) with 13 experimental runs was used to investigate the optimum conditions for the extraction, and the selected variables were solvent volume from 100mL to 300mL and extraction temperature from 100 ºC to 200 ºC. The maximum predicted Chlorogenic acids concentration (0.012 mg/mL) was obtained at the solvent volume and extraction temperature of 300mL and 200ºC respectively. Thus, the result from this extraction has been utilized for the formulation of hand sanitizer with green coffee beans extract. Evaluation of developed hand sanitizer was conducted by using disc diffusion method on the Escherichia Coli. An effective zone inhibition (13mm) of hand sanitizer was obtained when using green coffee beans extract at 300mL solvent volume and 200°C extraction temperature.