Equilibrium studies on adsorption of Bovine Serum Albumin (BSA) using PVDF membrane

PVDF Immobilon FL membrane has been acknowledged for potential use as a good material compatibility towards protein adsorption in biosensor application. To characterize the adsorption capability of membrane, equilibrium studies on adsorption isotherm is one of most useful method. In this study, the initial concentration of protein is varied in range 0.5 mg/L, 1.0 mg/L , 1.5 mg/L , 2.0 mg/L and 2.5 mg/L. The linear equilibrium isotherm model used to approximate with the experimental data are Langmuir type-I, Langmuir- type II, Langmuir type III, and Freundlich Isotherm. Preliminary for the experiment, the sample PDVF is been characterized in terms of the hydrophobicity, porosity, and surface polymorph by using water contact angle instrument, wetting liquid and weighting method, and Fourier transformation infrared spectroscopy (FTIR) respectively. The concentration of BSA adsorbed measured at λ = 562 nm by using UV-v is spectrophotometer.