Inhibitory effect of ethanolic extract of the germinated brown rice in 3T3-L1 adipocyte differentiation

Obesity has becoming an alarming issues to the worldwide concerns as this issues keep on increasing (Yun et al., 2010). Germinated brown rice (GBR) that has been reported to be good for digestion (Seung et al., 2015) and contain more nutritional value ( Patil et al., 201 l) which is good in controlling certain metabolic diseases. To study the effects of ethanolic extract of the germinated brown rice in 3T3-Ll adipocyte cell differentiation and to determine the optimal concentration of ethanolic extract of the germinated brown rice in decreasing the differentiation of 3T3-Ll adipocyte cell. GBR was extracted with ethanol and tested towards 3T3-LI cells and WRL 68 cells. The viability of both cells were undergo MTS assay and the absorbance was analysed by using microplate reader. 3T3-LI cells also undergo Oil Red O staining and the lipid contents was undergo MTS assay while lipid accumulation were observed under microscopic observation. All results were analyzed using one-way ANOVA. MTS assay revealed that the inhibitory concentration of 3T3-LI cells (IC50 ug/ml), whereas for WRL 68 cell (hepatic cell) the IC50 was at 250 ug/ml. Lipid accumulation was higher at adipocyte cell when compare to the adipocyte cell treated with ethanol extract and preadipocyte cell. This study demonstrated the suppression of the lipid accumulation when treated with ethanol extract of GBR. GBR may act as potential treatment and/or in prevention of obesity in the future