Biocatalytic productivity of alcohol dehydrogenase (ADH) in membrane bioreator: effect of pH: article

In the present study, immobilization enzymes are necessary for increasing the biocatalytic efficiency. At low pH during immobilization, the enzyme was liable to accumulate on the membrane surface due to the electrostatic adsorption and reduced the stability on enzyme. While when the solution of enzyme at high pH value (away from IEP) during immobilization, the membrane and enzyme was negatively charged and lead to much lesser accumulation on the membrane surface due to the strongest of electrostatic repulsion. Therefore, it will induce to lower fouling on the membrane. At the neutral pH (near IEP), the hydrogen bonding between enzyme and membrane was stabilized and lead to maintain the enzyme activity.