Extractions of two vitex species

Vitex species is known world widely as a medicinal plant and it has many therapeutical benefits. Vitex trifolia and Vitex negundo were selected for this research due to their availability and various chemical constituents. This research was performed to investigate the chemical constituents of Vitex trifolia and Vitex negundo by isolation, purification and identification of its chemical constituents. Isolation of the methanol and ethanol extracts were done by using Preparative Thin Layer Chromatography. The compounds of interest were subjected to purification in order to obtain only pure compounds, without the impurities. A pure compound was introduced to the Nuclear Magnetic Resonance (NMR) spectroscopy technique and 1 H­ NMR analysis was performed to identify its chemical structure. Finally, the chemical compound that was isolated from the leaves of Vitex trifolia was determined as agnuside.