Attachment of Pseudomonas aeruginosa NR.22 (Ps.NR.22) on ceramic for filtration of crude oil in water mixture

Pseudomonas aeruginosa NR.22 (Ps.NR.22) has a gram negative rod shaped bacteria was subculture from pure strain isolated by Nik Raikhan and Khairul Izwan that has a potential to degrade the crude oil which consisted of SARA component. Crude oil from Dulang Field was homogenized by using ultrasonic homogenizer. As to meet the first objective, Ps.NR.22 was attached to a ceramic membrane by using dead-end method. The second objective was observed and analysed by using comparison method between oil-water mixture that was filtered with no bacteria and with the presence of bacteria. The result was further examined by using ultraviolet-visible (UV-VIS) spectroscopy which degrade only by 22.4 % for the filtration without attachment of bacteria while percentage of degradation of hydrocarbon for the presence of bacteria is 77.6 %. As for the percentage between both product of filtration is 70.8 %. Another result was analysed by Fourier transform infrared spectroscopy (FTIR) which resulting in increment of degradation of crude oil from three bond to six bond for the filtration with the presence of bacteria. On the other hand, the morphological membrane characterization was done by using digital microscope as it is revealed that the bacteria were actually degraded the crude oil and thus, the surface of membrane was clearer compared to the product of ceramic membrane without bacteria. In conclusion, the oil-water mixture filtered with presence of bacteria was clearer compared to the product of ceramic membrane without bacteria. To achieve precise result, various species of bacteria need to be attached to the ceramic membrane in order to filter the crude oil to get the clearer water.