Evaluation of solvent and temperature on the yield of polyphenols in black tea

Camellia sinensis from the Theaceae family is commonly called tea and it is rich in antioxidants, polyphenols. This research deals with the plant, in which the main focus is to obtain the yield of total polyphenols from the leaves. The objectives of this study are to determine the effect of different types of solvents and different temperatures (60°C, 70°C and 80°C) on the yield of polyphenols. The dried leaves undergo Soxhlet extraction for 30 minutes by using 2 types of solvent systems which are ethyl acetate : ethanol (2:3) and ethanol : water (1: 1). The total phenolic content is determined by using Folin-Ciocalteau method in which gallic acid is used as a standard compound. The total phenolic content is calculated by using the gallic acid standard curve equation : y = 0.0003x + 0.008. All determination was performed in triplicate. For respective temperature at 70°C, the mixture of ethyl acetate : ethanol soluble sample gave the highest total phenolic content of (74.22 ± 10.61) mg/g.