Optimization of high performance liquid chromatography (HLPC) methods for the alcoholic extracts of pandanus

There are two main objectives of this research. The objectives include the review of various liquid chromatography methods and high performance liquid chromatography method's optimization for Pandanus species. The chromatography method is a method used to separate compounds that exist in a particular sample. Various liquid chromatography methods reviewed include the Thin Layer Chromatography (TLC), Medium Pressure Liquid Chromatography (MPLC), Vacuum Liquid Chromatography (VLC), High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC). The reviewed studies shows different separation of compound obtained from various liquid chromatography method performed. For the HPLC method optimization, there are three samples involved. Samples are subjected into reverse phase HPLC (RP-HPLC) for analysis are the Pandanus amaryllifolius, Pandanus monotheca and Pandanus sanderi. The samples were dissolved and filtered before introduced to the RP-HPLC for analysis. The best chromatogram can be obtained by optimizing the parameters involved in the analysis. Optimization of the methods on the parameters such as the flow rate, time of analysis, solvent's gradient system, and absorbance wavelength were done. The results were promising due to the presence of single isolated peak indicating presence of pure compound. Therefore, it is suitable to be further studied to improve the understanding on the Pandanus species.