Co-culture of fungi and bacteria

The aim of this study is to investigate the modulation of secondary metabolism of given the bacteria and fungi. Eleven species of fungi were selected based on their chromatogram profile as showed in the preliminary study. The fungi were GM29, CI08.3, CMOl, MEI 7, GS41, GS38, GM33, GS47, CR25, CM20 and CR15.2. Meanwhile the bacteria were collected from the frezer at RiND which has been identified by the researchers. The bacteria were Pantoea agglomeraus (1. Eg. 16), Klebsiella oxytoca (10. Pl. 3), Cronobacter sakazakii (1. Tp. 5), Pseudomonas putida (1. Eg. 6), Bacillus subtillis (1. B2. 2), Acinetobacter calcoaceticus (6. Le. 4) and Bacillus cereus ( l .Eg. 1 7). These fungi were studied for their bio-activities when being cultured together with several species of bacteria in the MTP plate. The MTP plate was incubated for two weeks which later on proceeded with HPLC profiling. Based on the HPLC analysis, it showed that some of the selected fungi have a potential in eliciting the secondary metabolite compounds through the co-culture with bacteria. However the identification of the compounds had not yet completed.