Effect of substrate concentration and reaction time of Aquilaria Malaccensis leave extract on inhibition of pancreatic lipase

Obesity has been arising from a day to day and has become a very complicated health issue worldwide that must be seriously taken. From the research, this complicated problem can be solved by inhibiting pancreatic lipase that will block the absorption of fat. Thus, the main focus of this research were identification of the presence of phenolic and flavonoid compound that can be derived from Aquilaria malaccensis leaves extract to inhibit the pancreatic lipase and determination of the effect of substrate concentration and reaction time of A. Malaccensis leaves extract on inhibition of pancreatic lipase. There were only Gallic acid and Quercetin compunds used in this study to determine the total phenolic and flavonoid contents respectively. This study was conducted in two different types of A. Malaccensis leaves which were dried and fresh leaves. Both types of leaves were pretreated by ultrasonication and then distilled through hydrodistillation by using a TOPS MS-6 heating mantle followed by evaporation using a Heidolph rotary evaporator. Finally, the presence of phenolic and flavonoid compound in A. Malaccensis leaves extract was analysed by using Total Phenolic Content (TPC) assay and Total Flavonoid Content (TFC) Assay respectively and also using High Performance Liquid Chromatography (HPLC). The parameters involved in the process were substrate concentration (100µM, 200µM, 300µM) and reaction time (20, 40, 60 minutes) during inhibition of pancreatic lipase process. From the result obtained, the value of TPC and TFC was higher in fresh leaves extract compared to dry leaves extract as the absorbance increase. Besides, the concentration of phenolic and flavonoid in fresh leaves extract by using HPLC was higher compared to dry leaves as the area of the peak in the graph was larger. Lasty, the percentage of inhibition was higher by utilizing lower substrate concentration from both dry and fresh samples as the value of absorbance increased.