Bisphenol A: degradation by pseudomonas aeruginosa isolated from local water source in Malaysia

A Pseudomonas sp. was isolated from lakes (Section 2 and Section 7) and sewer (SIRIM BERHAD), Shah Alam, Selangor by using Pseudomonas isolated agar for about 24 hours of incubation. There about 4 species of Pseudomonas sp. based on observation after 24 hours of incubation and it was found that one of the species which is species A was identified as Pseudomonas aeruginosa. This is because only Pseudomonas aeruginosa (species A) show green pigmentation on it and species B, C and D not showing any color of pigmentation. Thus, Pseudomonas aeruginosa was chosen based on the average reading of absorbance (540nm) as sole species for the growth kinetics studies. Growth kinetics studies on the Pseudomonas aeruginosa show the bacterial able to adapt in the nutrient broth containing BPA. The rough idea on how Pseudomonas aeruginosa degrades BPA is the bacterial cell will used carbon in BPA as one of carbon source. The morphology Pseudomonas aeruginosa was identified by gram staining method which the cells were in rod-shape and Gram negative.