Cytotoxicity effect on liver cancer cells and xanthine oxidase inhibitory activity of fruit peels extract / Nur Atikah Zulkefli

In this study the fruit peels extract consist of mango, honeydew and papaya was investigated to explore their cytotoxicity against liver cancer cell and xanthine oxidase inhibition activity in vitro. The total phenolic and total flavonoid content of the extract were also measured. For determination of cytotoxic property of extract against cells, both human hepatoma cell line HepG2 and human hepatic cell line Wrl66 cell lines were treated with extract and gallic acid as positive control which then incubated for 24 hours. MTT assay is used to determine the number of viable cells. The percentages of number of viable cells were measured by dividing absorbance sample with absorbance of control and multiply with 100%. The results showed that, extract give less cytotoxicity toward HepG2 cell as compared with gallic acid but higher cytotoxicity activity when compared with Wrl68 cell. In xanthine oxidase inhibition assay, allopurinol, gallic acid and quercetin were used to compare with extract. The results showed that, extract has quite the same value of inhibitory activity with allopurinol and quercetin toward xanthine oxidase but gallic acid has slightly lower inhibitory activity as compared with other. As a conclusion, fruit peels extract may have the potential to exhibit cytotoxic activity and able to inhibit xanthine oxidase. Their activity could be contributed by the total phenolic and flavonoids.