Amplification of MiRNA-34A from normal human liver (WRL 68) for neurogeneration study / Nur Ezzaty Rizdiana Che Ridzuan

The microRNA-34 (miR-34) is a family of three miRNA (miR-34a, miR-34b and miR-34c) and among the miR-34 family, miR-34a has gain an extensive interest in miRNA therapeutics of neurodegeneration (Di Martino et al., 2012). MiR-34a was said to affect neuronal function and morphology (Agostini et al., 2011) and reduce p53 regulation of induced pluripotent stem cell generation (Choi et al., 2011). This research was performed to identify and amplify the miR-34a sequence by designing primers, polymerase chain reaction (PCR) and sequencing analyses. The primers of MiR-34a were designed from NCBI Primer Blast and the components involved were subjected to PCR. The purified PCR product was analysed through sequencing analysis. The results from the sequence analysis signify that the miR-34a was successfully amplified.