Microbial metabolism of estriol with filamentous fungi / Azizah Yang Redzuan

Biotransformations is the process by which compounds transformed into other metabolites biologically while microbial transformation means that the compounds transformed into another metabolites biologically by using microorganisms such as fungi. In the present research, Trichothecium roseum was used to fermented bioactive steroid, Estriol. The main objective of our research was to observe the microbial transformation of steroidal molecules using filamentous fungi. Compound 1, Estriol was inoculated into Trichothecium roseum. The fermentation flask of estriol was then extracted after 3, 6, and 12 days of fermentation period. As a result of fermentation process, the transformed products of estriol have been investigated through TLC and HPLC methods. TLC method showed indication of metabolites production during fermentation while HPLC showed the peaks and retention time of transformed metabolites. Based on the results of TLC and HPLC methods, secondary metabolites were detected after 3, 6, and 12 days of fermentation. However, due to limited time, the characterization of transformed metabolites has not been studied.