Effect of PVDF membrane pore structure on protein immobilization: a study using immobilon – PSQ and immobilon – FL / Mohamad Sidiq Mohd Basir

Membrane study is one of the most important field that is related with the adsorption science and biomedical research. Membrane has many applications in industry such as waste water treatment, medical application that immunoassay and etc. There are many types of membrane with different characteristic that can be applied in similar application, but in order to know which type of membrane is suitable for the application, the membrane morphology must be study. Membrane morphology such as porosity, hydrophobicity, pore structure and etc. play an important role in the effectiveness of the membrane. This paper is evaluating the membrane pore structure on protein immobilization. The membrane that was used for this study is polyvinylidene fluoride (PVDF). Two different types of PVDF membrane were selected: immobilon – PSQ and immobilon – FL. This two types of membrane were study by comparing their morphology using FTIR, water contact angle and porosity test. Protein binding experiment was performed using the two membrane to compared the performance of protein adsorption of the two membrane. From the result, it can be concluded the morphology of different PVDF membrane can affect the amount of protein immobilazation to the membrane.