CO₂ removal using immobilized carbonic anhydrase on amberlite

Due to rapid emission of CO₂ in atmosphere, an approach has to be made to solve this issue. This paper proposed an approach which known as immobilization of carbonic anhydrase (CA) on amberlite for CO₂ removal. CA has been immobilized on amberlite by cross-linking method using glutaraldehyde. CA immobilized on amberlite was further tested in application of carbonation reaction which involved conversion of CO₂ to CaCO₃. It was observed that the optimum time for hydration process in carbonation reaction using immobilized CA was 10 min as compared to blank sample (15 min). The optimum mass of immobilized CA on the CaCO₃ precipitation was 0.4 g. The carbonate was characterized using XRD and FESEM to validate the formation of CaCO₃. FTIR instrument was used to determine the functional group (NH₃) present in immobilized CA enzyme structure. In conclusion, immobilized CA on amberlite support by cross-linking method could be an effective and economical in the practical conversion system of CO₂.