Bioremediation of hydrocarbon contaminated soils using locally P.Aeruginosa with organic and inorganic fertilizer: article / Siti Zulaikha Shaikuthali and Azzah Nazihah Che Abdul Rahim

The discharge of oily wastewater to environment caused serious damages to human, animal and environment and bioremediation are an attractive alternative to chemical method for removal of hydrocarbon from oily wastewater. Pseudomonas Aeruginosa were utilized in degradation of different hydrocarbon (kerosene and diesel oil) contaminated soils amended with inorganic (NPK and Urea) and organic (Shrimp waste and Egg Shell Powder) fertilizers and in some of their combination. The incubation period ranged from 3-16 days. Results showed that bacterial population count increased as the microbes’ utilized hydrocarbon for carbon and energy, residual hydrocarbon decreased was stimulated by the fertilizer and percentage degradation increased. Pseudomonas Aeruginosa degraded kerosene and diesel better in the presence of NPK fertilizers and urea fertilizer. More than 90% of the hydrocarbons were degraded with each incubation period. Bacterial population is keep increasing for first 15 days but after 15th day it begins to drop because of the secretion of toxic secondary metabolites. Furthermore, the usage of Pseudomonas Aeruginosa to degrade hydrocarbon has been proven as it can degrade kerosene and diesel.