Rhamnolipid NR.22 green detergent by Pseudomonas aeruginosa NR.22 / Fathiin Nabiilah Rahman

Washing detergent consisted of variety of components as example surfactants and enzyme. Green Alternative or biodegradable detergent has been introduced by using Pseudomonas aeruginosa NR.22 to produce green detergent rhamnolipid NR22. This research started with the preparation of rhamnolipid biodetergent and the chemical structures of the surfactants product was confirmed by Fourier Transform Infrared Spectrometer (FTIR). The results show 40% of foaming formation can be obtained and the foam could remain to 5 hours which qualified as the good stability product of biosurfactant. Absorption valleys at 3286.26 and 3308.81 cm-1 in commercial and produced biosurfactant respectively shows the O-H band bond as hydroxyl groups. Another broad absorption valley observed in the value of 2151.11 and 2159.97 cm-1 were demonstrated in stretching C-C bond which was in alkyl groups. Absorption at 1637.85 cm-1 for the commercial biosurfactant and 1634.89 cm-1 for the produced biosurfactant was characterized as stretching C=C bond of alkene groups. A bending of C=O bond at 1737.26 and 1365.16 cm-1 for produced biosurfactant were in a group of carboxylic acid. The wavelength of 1364.82 cm-1 for the commercial biosurfactant also came from the same group of carboxylic acid. The absorption at 1217.02 cm-1 for the produced biosurfactant consisted the stretching of C-O bond which in hydroxyl group. The percentage oil removal of 94.87% was excellent using the formulation of biodetergent which was nearly similar to the commercial detergent (99.5%) and mix of commercial detergent with biodetergent (96.6%). The trend of the graph of oil removal percentage versus temperature of washing medium shows as increasing in temperature of washing medium, the percentage of oil removal on the cotton cloth also increasing. Rhamnolipid NR22 have the capability and high potential as a good biosurfactant and detergent in comparing the images using Scanning Electron Microscopy (SEM).