Changes in the metabolism pathway of liver organ infected with Klebsiella pneumoniae / Adila Hani Mohd Nazari

Klebsiella pneumoniae is one of the most common multidrug resistant bacteria. One of the reasons is increased number of extended-spectrum beta-lactamases produced by the bacteria. With metabolomics, new drug targets can be discovered to treat bacteria that are capable of multiple drug resistance enzymes. This study aims in profiling the changes in the metabolism of the K. pneumoniae infected liver organ using LC/MS­ QTOF and to identify metabolism pathway involved in the infected liver organ. This study is done by using normal and K.pneumoniae infected liver organ of Rattus norvegicus. The organs were cut to get similar weight and solidified using liquid nitrogen. Then, the organs were grinded to tum them into powder. Several solvents were used to extract the sample; such as methanol, chloroform, acetonitrile and distilled water. The samples were injected into LC/MS-QTOF for analysis. MetPa; a web-based tool is used to analyze and visualize metabolic pathway. Based on the metabolic profiling and metabolic pathway analysis, liver organ infected with K.pneumoniae showed changes in two metabolic pathways which include retinol and arachidonic acid metabolism. The role of retinoic acid in retinol metabolism pathway in infection is to promote differentiation of T and B cells, thus induce immune tolerance while, leukotriene A4 is an intermediate of a potent chemotaxis. The presence of retinoic acid and leukotriene shows they may be involved in K.pneumoniae infection in liver organ. Therefore, validation is required so that it can be use as biomarker in Klebsiella pneumoniae diseases.