In Vitro study of Hydroxyapatite (HAp) specimen in the Simulated Body Fluid (1.5SBF): article / Ainina Kamalrulzaman and Dr. Istikamah Subuki

This study had been conducted to determine In-Vitro properties of hydroxyapatite (Hap) pellets synthesize from the clamshells via precipitation method. HAp pellets were successfully prepared and then immersed in the simulated body fluid (SBF) which has a similar composition to the human blood plasma. Therefore, 1.5SBF concentration has been chosen as fluid for InVitro studies. This research also highlights the purpose of In-Vitro studies of HAp powder in order to verify the formation of apatite layer on the surface of HAp pellets after immersed in the simulated body fluid in certain period of time up to 25 days. After immersion, the HAp pellets are then characterized by using Field Scanning Electro Microscopy (FESEM) with Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transform Infrared Spectroscopy (FTIR). The Fourier Transform Infrared Spectroscopy (FTIR) shows the intensity of functional groups in the Hap such as phosphate, carbonate and OH groups. While, by using Field Scanning Electro Microscopy (FESEM) with Energy Dispersive Xray Spectroscopy (EDX), the morphology of apatite layer formed in the surface of Hap pellet was being analysed and studied. It is shows the growth of apatite layers formed in the surface of HAp after immersed in the 1.5SBF.