Determination of the metabolite profiles of Klebsiella pneumoniae infected spleen organ by LC/MS-QTOF / Normi Asmida Rasid

Spleen of Rattus norvegicus was used in order to identify the metabolite profile of the normal and the infected rats with Klebsiella pneumoniae. During the infection, many metabolite pathways may be involved in the pathogenesis of Klebsiella pneumoniae infection. This study was conducted using the advanced technologies of chromatography, LC/MS-QTOF. Six samples of normal and infected rats were prepared. For the extraction, the organs were grounded, then mixed with extraction solvent which is water: methanol: chloroform with ratio 2:4:4 respectively. The mixtures were then centrifuged and dried using concentrator. After that, the samples were injected into the LC/MS-QTOF. Using the Agilent Metabolomic Discovery software, analysis was done by transferring the data from the LC/MS-QTOF into the software. The result showed that there are significant metabolites and pathways involved in the pathogenesis of Klebsiella pneumoniae infection. In the 2D and 3D PCA, different metabolites were expressed by the control and infected groups. The up and down regulation of the metabolites in the normal and infected condition were further analysed. The significant pathway involved in the study are retinol and tryptophan pathways. Retinol are essential for growth and cell differentiation, particularly of epithelial tissue and tryptophan metabolism under pathological conditions is a powerful mediator of responses in both the innate and adaptive immune systems. Therefore, by knowing the pathways involved using pathway analysis, it provides clues to the researchers to strategize approaches to prevent and treat hosts or patients with spleen infected with Klebsiella pneumoniae.