Annona Muricata : extraction of AGEs from leaves / Muhammad Eiman Uzmi Shaari

Since the dawn of medicine, natural products, especially those derived from plants, have been used to help mankind sustain their health. In the last century, phytochemicals in plants have been a pipeline for pharmaceutical discovery. The importance of plant active ingredients in agriculture and medicine has stimulated considerable scientific interest in the biological activities of these substances. Plants with a long history of use in ethno- medicine are a rich source of active phytoconstituents in a pharmaceutical landscape that offer medicinal or health through benefits against various diseases. Annona Muricata is also commonly referred to as Gunbanana Graviola or Soursop. Therefore, the aim of this work is to study the effect of solvent concentration towards the extraction of bioactive Acetogenin compounds in Annona Muricata . Extraction involved during experiment was liquid – solid extraction. The Annona Muricata leaves were picked and collected for about 500 gram were shade dried and powdered. The powder was macerated with 0 - 95% ethanol and water to obtain six different fraction F1-F6 which were bringing into analysis. HPLC and FTIR analysis showed that with different combination of water – ethanol concentration added into fractions affect the presence of acetogenin compounds and chemical groups. Four acetogenin were found in HPLC analysis. Besides, each fractionation showed different level of chemical groups presence in FTIR analysis.