Performance of laccase Ps. NR22 in crude oil degrading activity: kinetic study / Nur Amirah Zaharim

Due to the hazardous and toxic chemicals which contaminated soil, ground water, sediments, surface water and air give unfavourable effect to our ecosystem. Therefore, microbial degradation enzyme based is introduced in this study. UV visible and Gas Gravimetric Mass Spectrometry (GC-MS) were used to analyse this research. Also, Michealis Menten is a mathematical model used to prove the objectives. At the end of this study, bacterial laccase has the ability to degrade 70% of hexane, one of the components in the crude oil. As conclusion, performance test of bacterial laccase based on kinetic study and ability are proven. From the results obtained, the reaction velocity for the laccase to degrade crude oil is 1.0986 mole/h in 24 hours. Hence, the objectives were achieved. In future, performance test by using different types of bacterial enzyme on asphaltenes component should be done since asphaltenes were major fraction composition in crude oil.