Amplification of microRNA-17 and microRNA-18 sequencefrom HepG2 cancer cell line / Izzati Syahirah Sapuan

PCR is a rapid and modem technique for amplifying DNA sequences, which is based on the principle of enzymatic replication of the nucleic acids (Stanek, 2013). HepG2 is a human liver cancer cell which develop from chronic diffuse liver disease, other disease like Hepatitis B or Hepatitis C or from other triggers like chronic alcohol consumption, environment or by any harmful chemical. In HepG2 cancer cell, there is an over expression of miRNA-17 and miRNA-18 which enhance the migration and proliferation of the liver cancer cell (Sun et al., 2013). The present study was conducted to determine whether the sequence of the miRNA-17 and miRNA-18 in the HepG2 cancer cell line can be amplified by using the specific primer. The sequence alignment of miRNA-17 and miRNA-18 could be seen clearly by using BOXSHADED software even though there are two base pair in sequence of miRNA-18 that are different from the original sequence. This is due to the substitution of G to C and point mutation ofN. In conclusion, the sequence ofmiRNA-17 and miRNA-18 of HepG2 cancer cell line can be amplified by using the specific forward and reverse primer.