Cytotoxic and antimicrobial activities of mixed natural product (trigonella foenum graecum, aloe barbadenis miller and cocos nucifera) aqueous extract against human colorectal cancer cell line and urinary tract infection causing bacteria / Nurul Farhana Akan Ajam @ Khaizan

Colorectal cancer (CRC) is one of the common causes of death worldwide. Urinary Tract Infection (UTI), on the other hand, is one of the most prevalent infectious diseases. Drug resistance is currently one of the obstacles that compromise the treatment of these diseases. Given the increasing importance of natural product in drug discovery, therapeutic agents from natural source may serve as good alternatives to current treatment. The present study evaluated the pharmacological effect of a natural plant extract, Trigonelline Foenum Graecum (TFG) seeds supplemented with coconut water (CW) and Aloe vera (AV), against UTI causing pathogens and CRC cell line (HCTl 16). The method used to test the antimicrobial and cytotoxic activities were Disc Diffusion Method and SRB assay, respectively. For antimicrobial activity, the freeze dried TFG+CW+AV extract showed better antimicrobial activity than the boiled counterpart. Ground TFG seed showed no antimicrobial activity even at the highest concentration tested. Two bacteria strains were found to be resistant to TFG+CW+ AV extract were E. coli and S. typhi. The extract did not show selectivity towards Gram negative or Gram positive bacteria. In cytotoxic study 5-FU was the most potent with IC50 of 0.0006 ± 0.0004mg/rnl. The boiled extract showed slightly better potency when compared to ground extract with IC50 of 0.025 ± 0.0019mg/ml and 0.029± 0.014mg/ml, respectively. Therefore, it is recommended that future study should be conducted to improve the accuracy and consistency of the result by using different method of extraction or different solvent.