Biotransformation of ethinylestradiol, andrographolide, tibolone and 16, 17-epoxyprogesterone using pereskia bleo in the vitro culture / Nur Aina Ameerah Che Mokhtar

Pereskia Bleo which is also known as Pokok Jarum Tujuh Bilah in Malay populationis a member of the genus Pereskia from the family Cactaceae. This plant is popular in the traditional treatment because it was stated that it is beneficial in treatment of some serious disease likes cancer, high blood pressure, diabetes and some inflammation problem. Due to its medicinal properties and also their wide availability, this plant was chosen as the resources for the biotransformation process. Several compounds including ethinylestradiol, andrographolide, tibolone and 16, 17- epoxyprogesterone were fermented with the callus of the Pereskia bleo. The media use for initiation of callus was Murashige & Skoog (MS) media which were implemented with the 6-benzylamino purine (BAP) and 1-naphthylacetic acid (NAA) hormones. The HPLC-DAD and HPLC-ELSD was used identify the biotransformed product. From the result, additional peaks with different elution times were identified in the HPLC profile of sample containing callus with the tibolone after compared with positive (media + tibolone) and negative control (media + callus). As a conclusion, fermentation of the P.bleo callus with tibolone was able to undergo biotransformation process.