Amplification of the purine-rich region from downstream of mirna17-92a sequence for triple-helix study / Norf Arahin Norhisam

Most of the cancer treatments are invasive and have other adverse effects to the cancer patient. The triplex-forming oligonucleotide (TFO) can be the alternative way to treat cancer patient without harm their body. In this research, the triplex forming oligonucleotide (TFO) sequence downstream to the miRNA 17-92a cluster in HepG2 cancer cell lines had been amplified as the initiation step in constructing the study on the TFO in hepatic cancer cells. The TFO binding site is too small to get the good band's view on the gel, therefore the conditions of gel electrophoresis also being optimized.