Determination of antioxidant activity of scidff bases synthetic compounds by using ferric reducing antioxidant power (FRAP) assay / Norfashihah Abdul Samad

The use of antioxidant in daily life can give many beneficial effects. It can scavenge free radicals released by oxidation and thus protects the cell from damage cause by the free radicals. Antioxidants can be found in both natural and synthetic compound. The Schiff bases synthetic compounds are an important class of organic compound that have intensive range of biological activities and industrial application. They have been found to have many beneficial pharmacological activities such as antibacterial, antifungal, anticancer, antiHIV, antitubercular, antiviral, anti-inflammatory and others. This study is conducted to determine the antioxidant activity of Schiff bases synthetic compounds by using ferric reducing antioxidant power (FRAP) assay. The aim of this study is to detect how many compounds from 27 Schiff bases synthetic compounds that will give high antioxidant activity by using FRAP assay. The principle of FRAP assay is antioxidant activity will reduce Fe3+ to Fe2+ that will develop an intense blue color. The Fe2+ formed may interact with 2,4,6-tris(2pyridyl)-s-triazine (TPTZ) and will give a strong absorbance at 593 nm. 27 Schiff bases synthetic compounds have been tested using this assay with the use of gallic acid as the positive control. FRAP value of the samples are measured and compared with the FRAP value of gallic acid to determine the potential of antioxidants activity of the compounds.