Development of a PCR based method for detection of genetic polymorphism of MLH1 in colorectal cancer / Norlida Che Yaacob

Colorectal cancer (CRC) is the second most common disease that relate to cancer in most developed countries. The loss of normal mismatch repair functions causes a mutator phenotype which is indicated by changes in the length of microsatellite sequences that results in abnormality of genome. Germline mutations in the DNA mismatch repair (MMR) genes hMLHI are responsible for contribution of hereditary nonpolyposis colorectal cancer (HNPCC). MutL homolog l(MLHl), a colon cancer­ related human gene which is found on Chromosome 3, is a gene that is commonly related with HNPCC. This gene has been identified as a locus that is commonly mutated in HNPCC. In this study, development of polymerase chain reaction based method was conducted and successfully developed and validated. A two-steps PCR method was successfully developed.