Cytotoxicity of nitric oxide in triple negative breast cancer cell (MDA-MB-231) / Fathin Abdul Halim Zaki

Breast cancer (BC) has three types and one of it is triple negative breast cancer (TNBC). MDA-MB-231 is one of the cell line in TNBC. There is no specific drug used to treat TNBC. Nitric oxide (•NO) is used to treat hypertensive emergency and congestive heart failure. In this study, •NO released by Sodium Nitroprusside (SNP) was studied. We hypothesized that •NO was more cytotoxic in micromolar concentration in comparison to tamoxifen. Tamoxifen is a well-known drug to treat BC and one of the drugs used in the treatment of TNBC. The result showed that the exposure of MDA-MB-231 cells to a range of SNP concentrations was unable to cause cytotoxicity ofMDA-MB-231 cells. A minimum cell death upon SNP treatment was observed at 25 µM after 24 h of treatment. It can be concluded that SNP at low concentration can be more cytotoxic to MDA-MB-231 cells.