Effects of tinospora crispa callus extract on liver cancer (Hep G2) cell line / Shazana Daud

Tinospora crispa is a climbing shrub that is usually grown in the Asian tropical region. T crispa plant has claimed to have many benefits and has been used for many therapeutic purposes such as antidiabetic and antihypertensive. A previous study also showed that T crispa natural plant has anticancer activity towards human cancer cell lines such as breast and liver cancers. So far, no research was done on investigating the anticancer properties of T crispain vitro cultures. Therefore, this study was conducted to investigate the effect of T crispa callus against liver cancer cell lines (Hep G2) since the plants grows faster using propagation technique. T crispa callus growth was induced by using eight different concentrations medium that contain different concentrations of 6- benzylaminopurine and was supplemented with fixed concentration of 1-napthalene acetic acid. The results of this study showed that the T crispa callus was able to grow, however the callus die after subculture process. This might be due to exposure to the contamination during subculture procedure. The callus used for treatment against liver cancer cell was selected only from certain concentration of media that are media B (MS+0.5mg/L BAP+ 0.25mg/L NAA), F (MS+4.0mg/L BAP+ 0.25mg/L NAA), G (MS+6.0mg/L BAP+ 0.25mg/L NAA) and H (MS+8.0mg/L BAP+ 0.25mg/L NAA). The liver cancer cells (Hep G2) was then treated with different extract concentration of T crispa callus that is combined from selected medium concentration which then incubated for 96 hours. MTT assay was carried out and the plates were then read by plate reader. The results obtained showed that the higher concentration of treatment, the lower percentage of cell death. However, the reliability of data needs to be improved since only one replicate was done to test on Hep G2. Further study should be done to obtain more reliable result.