Blood-based bio markers for early detection of alzheimer's disease / Maisarah Ahmad

Alzheimer's disease (AD) is one of the most common forms of dementia. Finding a biomarker for early detection of AD based on blood source has been a goal of researchers for many years. Studies have proven about 80% of human gene expression are found in the blood. We performed quantitative real-time polymerase chain reaction (RT-PCR) to determine the relative expression of gene in AD compared to healthy subjects by using mathematical model called Pfaffl method. Based on the Pfaffl method, we found that the relative expression of mitogen­ activated protein kinase (MAP2Kl) was higher relative to healthy subjects based on fold change ratio after normalization with reference gene, beta-actin (P-actin). This study provides evidence that blood can be a source of biomarkers for early detection of AD and thus can give an impact in terms of clinical practice, and ultimately may improve the patient's quality of life.