Confocal microscopic analysis of human colorectal adenocarcinoma cell lines (HT-29) against banana soft pith ethyl acetate extract / Sharifah Shazana Syed Ali

Colorectal cancer (CRC) has becomes the second most common cancer in Malaysia. There are numbers of treatment available for CRC such as chemotherapy, radiation therapy and surgery. However, those treatments have various side effects that reduced their quality of life. Thus, the drug development on natural products is crucial in order to reduce the side effect of modern therapies. This study was done to morphologically identify the mode of cell death induced by ethyl acetate extract Musa Paradisiaca cv Awak against colorectal cancer adenocarcinoma cell lines (HT-29) using confocal view of unstained sample and AO/Pl staining. Both confocal view of the unstained sample and AO/Pl staining were done for 24, 48 and 72 hours of incubation time by using various concentrations which are 0, 50, 100, 250, 500 and 1000 µg/ml. The view of unstained sample displayed the distribution of the cells after treated with the banana soft pith extract, whereas the cells stained with AO/Pl stain showed the cells undergo morphological changes such as chromatin condense, cell shrinks and membrane blebbing which indicates the apoptotic events.