Cytotoxic and antimicrobial activities of mixed natural product (trigonella foenum graecum, cocos nucifera and aloe barbadenis) ethyl acetate extract against urinary tract infection causing bacteria and human colorectal cancer cell lines / Siti Najihah Sa'don

Trigonella Foenum Graecum (TFG) is a family member of Fabaceae group. Chemical compounds from this plant were known to exhibit antimicrobial and cytotoxic activities. The present study investigated the cytotoxic and antimicrobial effect induced by ethyl acetate extract of TFG supplemented with coconut water (CW) and Aloe Vera (AV) against colorectal cancer (CRC) cells (HCTI 16) and against Escherichia coli, P. aeruginosa, S. aureus and S. typhi and B. subtilis. The antimicrobial activity was accessed after 48 hours of incubation by using disk diffusion method. Meanwhile the anticancer effect of this extract was accessed using sulforhodamine assay after treatment and cultured for 72 hours. For comparison purpose, ground TFG extracts and positive controls (antibotics for antimicrobial study and 5-FU for cytotoxic assay). For antimicrobial activity, ethyl acetate extract of TFG+CW+AV exhibited antimicrobial effect only against 2 Gram positive and 1 Gram negative bacteria. Meanwhile 2 Gram negative bacteria, S. typhi and E. coli show no sensitivity. In SRB assay of this extract inhibited HCTl 16 inn a dose dependent manner. This extract shows better antimicrobial and cytotoxic activity than ground TFG and the positive control have better action for both antimicrobial and cytotoxic activity among all the extracts. However, ethyl acetate TFG+CW+AV extract shown a better action against human colon cancer Further studies using different solvents should be explored to harness the full antimicrobial and anticancer potential ofTFG+CW+AV.