Neuroprotective effect of piracetam against LPS-induced neuroinflammation using EOC-20 cell: oxidative stress / Nur Farahanim Mohamad Asroh

Piracetam is a nootropic drug which acts as memory enhancers, neuron enhancers as well as cognitive enhancers. Piracetam has broad indication. It can be used to treat dementia, vertigo, sickle cell anemia and also as well as Alzheimer Disease (AD). The exact mechanism of action of this drug is still unknown so that many studies were conducted to determine it. In this study, piracetam was used to determine the neuroprotective effect against neuroinflammation. The microglial (EOC-20) cells were pre- treated with different concentration of piracetam (0.01, 0.1, 1, 10 and 100 µg/ml) before induced with LPS. The effects of treatment were compared between concentration, control and LPS alone. Griess assay and ROS assay were conducted and showed the decreased in nitric oxide (NO) and reactive oxygen species (ROS) level. DPPH assay also was conducted and showed that piracetam had mild antioxidant activity. In conclusion, high concentration of piracetam was approved of had the ability to protect cell from oxidative stress due to its antioxidant properties. Therefore, piracetam do exihibit neuroprotective effect on LPS- induced EOC-20 cell.