Qualitative analysis of banana soft pith crude extract anti-proliferative properties against oestrogen positive breast cancer cell lines (MCF-7) / Siti Suliza Wani Mohd Hanafi

Apoptosis is a programmed cell death also known as cellular suicide. Mostly cancer cells have loss the ability to induce apoptosis and caused excessive proliferation. Previous study showed that crude extract from Musa x Paradisiaca cv Abu was found to produce cytotoxic effect against oestrogen positive breast cancer cell-line, MCF-7. The present study was able to identify some typical morphological changes of apoptosis in BSP treated MCF-7 cell lines. In AO/PI staining method, treated cells (50, 100, 250, 500, 1000 ug/ml) shown morphological changes such as membrane blebbing, nuclear fragmentation, chromatin condensation and cell shrinkage. Further validation using TEM (IC50) was used to enhance the visualization of such changes. Similarly, membrane blebbing, chromatin condensation, nuclear fragmentations were observed. In addition apoptotic body, vesicles and vacuoles formation were clearly distinguished. These characteristics have strongly associated with apoptosis. This study has yet to prove that crude extract from Musa x Paradisiaca cv Abu possess potential anticancer properties. Further investigation are required to support the finding