Qualitative analysis of banana soft pith crude extract anti-proliferative properties against oestrogen positive breast cancer cell lines (MCF-7) / Siti Suliza Wani Mohd Hanafi

Mohd Hanafi, Siti Suliza Wani (2014) Qualitative analysis of banana soft pith crude extract anti-proliferative properties against oestrogen positive breast cancer cell lines (MCF-7) / Siti Suliza Wani Mohd Hanafi. Degree thesis, Universiti Teknologi MARA (Kampus Puncak Alam).

Abstract

Apoptosis is a programmed cell death also known as cellular suicide. Mostly cancer cells have loss the ability to induce apoptosis and caused excessive proliferation. Previous study showed that crude extract from Musa x Paradisiaca cv Abu was found to produce cytotoxic effect against oestrogen positive breast cancer cell-line, MCF-7. The present study was able to identify some typical morphological changes of apoptosis in BSP treated MCF-7 cell lines. In AO/PI staining method, treated cells (50, 100, 250, 500, 1000 ug/ml) shown morphological changes such as membrane blebbing, nuclear fragmentation, chromatin condensation and cell shrinkage. Further validation using TEM (IC50) was used to enhance the visualization of such changes. Similarly, membrane blebbing, chromatin condensation, nuclear fragmentations were observed. In addition apoptotic body, vesicles and vacuoles formation were clearly distinguished. These characteristics have strongly associated with apoptosis. This study has yet to prove that crude extract from Musa x Paradisiaca cv Abu possess potential anticancer properties. Further investigation are required to support the finding

Metadata

Item Type: Thesis (Degree)
Creators:
Creators
Email / ID Num.
Mohd Hanafi, Siti Suliza Wani
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Abdul Hamid, Hisyam
UNSPECIFIED
Subjects: R Medicine > RC Internal Medicine > Cancer
R Medicine > RS Pharmacy and materia medica > Pharmacopoeias
R Medicine > RS Pharmacy and materia medica > Materia medica > Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Pharmacy
Programme: Bachelor of Pharmacy
Keywords: banana, anti-proliferative, oestrogen, breast cancer cell lines (MCF-7)
Date: 2014
URI: https://ir.uitm.edu.my/id/eprint/111403
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