Development of PCR method for detection of genetic polymorphism of cytotoxic t-lymphocyte-associated protein 4 (CTLA4) gene in type 1 diabetes mellitus (TIDM) / Soraya Rosu

Type 1 Diabetes Mellitus is a condition in which the p cells that produce insulin undergo autoimmune destruction. It is associated with susceptibility gene of HLA region as well as non-MHC loci for example CTLA4 gene. NG transition at position 49 of exon 1 of the CTLA4 gene results in threonine to alanine substitution and was reported to have association with Type 1 Diabetes Mellitus. Objective of this study is to develop genotyping method for polymorphism detection of CTLA4 gene with Type 1 Diabetes Mellitus. Primers were designed and conditions for PCR were manipulated to obtain the most optimized PCR condition. 151 PCR was run at annealing temperature of 55°C and the intensity of the bands is higher as compared to annealing temperature of 58°C. Both wild type and mutant type bands appeared at different densities during the 2°d PCR analysis for all the five samples. More studies on association of CTLA4 gene with Type 1 Diabetes Mellitus are needed especially on Malaysian population as early detection is able to slow down or even stop disease progression and hence improve their quality of life.