The use of zn²⁺-cyclen lumazine as a molecular probe for detection of glutathione (GSH) or glutathione disulfide (GSSG) / Najwa Fatihah Mohd Noor Rawi

Mohd Noor Rawi, Najwa Fatihah (2014) The use of zn²⁺-cyclen lumazine as a molecular probe for detection of glutathione (GSH) or glutathione disulfide (GSSG) / Najwa Fatihah Mohd Noor Rawi. Degree thesis, Universiti Teknologi MARA (Kampus Puncak Alam).

Abstract

The significance of this study is to design a simple and selective molecular probe for detection of glutathione or glutathione disulfide. Zn²⁺-cyclen coordinated lumazine has been chosen to act as the probe in this study. Zn²⁺-cyclen complex which act as the receptor for lumazine will significantly enhanced the fluorescence intensity of lumazine. Glutathione which is a non-fluorescence compound will displace lumazine to coordinate with the Zn²⁺-cyclen complex due to the sulphydril group in its moiety that has higher affinity towards Zn²⁺ as compared to lumazine. The ability of glutathione to coordinate with the Zn²⁺-cyclen complex is indicated by the decrease in the fluorescence intensity of lumazine. There are only two major steps in order to synthesize the probe. The first step is the extraction of cyclen tetrahydrochloride salt into free cyclen. The second step is to do the complexation of free cyclen with Zn²⁺ by using Zn(NO₃)₂.6H₂O. The interaction of lumazine and glutathione with Zn²⁺-cyclen complex was measured by using fluorospectrometer. However, the selectivity of the probe towards glutathione was not determined due to limitations in chemicals available and time constraint.

Metadata

Item Type: Thesis (Degree)
Creators:
Creators
Email / ID Num.
Mohd Noor Rawi, Najwa Fatihah
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Mat Jusoh, Mohd Zulkefeli
UNSPECIFIED
Subjects: R Medicine > RS Pharmacy and materia medica > Materia medica > Pharmaceutical chemistry
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Pharmacy
Programme: Bachelor of Pharmacy
Keywords: zn²⁺-cyclen, molecular probe, glutathione (GSH), glutathione disulfide (GSSG)
Date: 2014
URI: https://ir.uitm.edu.my/id/eprint/111118
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